Temperature dependence of unbinding forces between complementary DNA strands.
نویسندگان
چکیده
Force probe techniques such as atomic force microscopy can directly measure the force required to rupture single biological ligand receptor bonds. Such forces are related to the energy landscape of these weak, noncovalent biological interactions. We report unbinding force measurements between complementary strands of DNA as a function of temperature. Our measurements emphasize the entropic contributions to the energy landscape of the bond.
منابع مشابه
Stretching and breaking duplex DNA by chemical force microscopy.
BACKGROUND Specific interactions between complementary strands of DNA and other molecules are central to the storage, retrieval and modification of information in biological systems. Although in many cases the basic structures of duplex DNA and the binding energetics have been well characterized, little information is available about the forces in these systems. These forces are of critical imp...
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ورودعنوان ژورنال:
- Biophysical journal
دوره 82 1 Pt 1 شماره
صفحات -
تاریخ انتشار 2002